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KMID : 0351619690100010013
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Kyungpook Medical Journal
1969 Volume.10 No. 1 p.13 ~ p.30
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Studies on the Growth Characteristics on the Trichophyton Differential Media and Amino Acid Utilization of Keratinophilic Fungi Isolated from Soil
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Abstract
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For the purpose of determining the growth characteristics and nutritional utilization of keratinophilic fungi of the soil origin, the author has isolated four species of fungi such as M. gypseum, chrysosporium sp. , K. ajelloi and M. coolcei from various sources and these strains were inoculated into both Georg´s trichophyton differential media and various kinds of amino acid added ammonium nitrate agar, and then observed growth characteristics such as colonial morphology, growth rate and pigmentation, with the survey o: size and morphology of macroconidia forme3 on various media.
1. Thy four kinds of keratinophilic fungi grown on thiamine, inositol sad nicotinic acid casein agar revealed luuriant growth macroscopically, but ammonium nitrate and histidine ammonium nitrate agar showed scanty growth.
2. M. gypseum grown on thiamine and inositol casein agar revealed abundant pleomorphic growth. chrysosporium sp. grown on nicotinic acid casein agar exhibited scant pleomorphic growth.
3. K. ajelloi and M. cookei formed pure pawderly colonies on all differential media.
4. Among 42 strains of chrysosporium sp. 14 strains (33.30 formed powderly colonies re,;ardless of composition of medium. The other strains are variable in colonial appearance.
5. The reverse color of 21 strains of K. ajelloi, appeared deep dark violet color of 20 strains grown on thiamine casein agar and crimson co:~or of the all strains grown on casein agar and inositol casein agar while no color change of 13 strains grow!i on ammonium nitrate agar an~i variable on Sabour and media.
6. All 14 strains of M. cookei ´grown on thiamine casein agar shoEVd crimson on reverse color howevar no r_rimson color formed on ammonium nitrate agar.
7. Although amino acid utilization is variable according to a kind of k;,ratinophilic fungi, M. gypseum grown on valine, chrysosporium on valine and serine, K. ajelloi on cystine, serine and valine, and M. cookei on isoleucine anti phenylalanine revealed poor growth.
8. Chrysosporium, K. ajelloi and M. cookei grown on alanine and M. gypseum and K. ajelloi on methionine revealed poor growth.
9. Relationship between nutrition and size of macroconidia:
1) M. gypseum grown on thiamine, inositol and nicotinic acid casein agar increased in its size.
2) K. ajelloi grown on inositol and nicotinic acid casein agar increased in its size while the growth on ammonium nitrate agar decreased in its size.
3) M. cookei growr. on all differential media decreased in its size.
10. Relationship between various amino acid and size of macroconidia showed that four kinds of keiatinophilic fungi grown on alanine, tyro-sine and leucine increased in its size.
1) 11rl.;gypseum grown on threonine increased mostly in its size.
2) Although K. ajelloi grown on all kinds of amino acid except glutamic acid increased in its size and most remarkable on alanine.
3) M. cookei grown on phenylalanine increas~d most in its size while grown on leucine mostly decreased.
11. There was no correlation between macroscopic colonial morphology of the various differential agar and amino acid added agar and size of macroconidia.
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KEYWORD
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